Anti-cancer and anti-inflammatory effects elicited by short chain fatty acids produced by Escherichia coli isolated from healthy human gut microbiota

Abstract Background Extracellular metabolites of short chain fatty acids (SCFA) excreted by gut microbiota have been reported to play an important role in the regulation intestinal homeostasis. Apart from supplying energy, SCFA also elicit immune stimulation animal and human cells. Therefore, attempt was conducted isolate producing bacteria healthy microbiota. The anti-cancer anti-inflammatory effects extracellular individual SFCA were further investigated using breast, colon cancer macrophage Toxin, inflammatory cytokine gene expressions RT-qPCR analyses this study. Results Escherichia coli KUB-36 selected study since it has capability produce seven extracellularly. It produced acetic acid as main SCFA. is a non-exotoxin producer hence, safe IC 50 values indicated that E. treatment elicited more potent cytotoxicity effect on MCF7 breast cell compared leukemia cells but exhibited little cytotoxic normal cell. Furthermore, could affect responses lipopolysaccharide-induced THP-1 they suppressed cytokines IL-1?, IL-6, IL-8 TNF-? well control, whilst inducing IL-10 expression. Conclusion possessed vast potential beneficial microbe activity simultaneously. However, probiotic characteristic should be elucidated vivo models.